Johne’s Disease Diagnostics Market - Global Industry Size, Share, Trends, Opportunity, and Forecast, Segmented By Animal Type (Cattle, Goat, Sheep), By Test Type (ELISA, PCR, AGID, Others), By End User (Veterinary Hospitals & Clinics, Others), By Region and Competition, 2021-2031F

Forecast Period	2027-2031
Market Size (2025)	USD 675.66 Million
CAGR (2026-2031)	6.53%
Fastest Growing Segment	ELISA
Largest Market	North America
Market Size (2031)	USD 987.55 Million

Market Overview

The Global Johne’s Disease Diagnostics Market will grow from USD 675.66 Million in 2025 to USD 987.55 Million by 2031 at a 6.53% CAGR. The Global Johne’s Disease Diagnostics Market comprises analytical assays and testing platforms, such as enzyme-linked immunosorbent assays (ELISA) and polymerase chain reaction (PCR), designed to detect Mycobacterium avium subspecies paratuberculosis in livestock. The primary driver accelerating market growth is the critical necessity to mitigate production inefficiencies, specifically reduced milk yields and premature culling associated with infected herds. Furthermore, rigorous herd health certification schemes and trade protocols necessitate frequent surveillance, compelling producers to invest in regular screening. According to Animal Health Ireland, in 2024, the estimated annual economic cost of the disease to the Irish dairy industry alone ranged between €11.24 million and €23.85 million, highlighting the severe financial implications that drive the demand for effective diagnostic protocols.

A significant challenge hindering the broader expansion of this market is the limited sensitivity of current diagnostic tools during the disease's lengthy subclinical phase. Infected animals often shed low levels of bacteria or produce undetectable antibody concentrations for years, leading to false-negative results that complicate eradication strategies. This technical limitation regarding early detection can diminish producer confidence in screening efficacy, consequently delaying the universal adoption of comprehensive testing regimes across cost-sensitive livestock operations.

Key Market Drivers

Technological advancements in rapid molecular and serological diagnostics are fundamentally reshaping the market by addressing the critical need for speed and sensitivity in detecting Mycobacterium avium subspecies paratuberculosis. The emergence of phage-based technology and high-throughput PCR now allows for the identification of viable bacteria much faster than traditional culture methods, which historically required months to yield results. According to Animal Health Media Journal, November 2024, in the 'New test detects Johne’s disease in day-old calves' article, a newly developed diagnostic system identified live infection at least four weeks earlier than standard fecal PCR in 75% of trial cases. Such innovations are vital for breaking the chain of transmission, enabling producers to isolate infectious animals before they contaminate the herd environment and compromise production efficiency.

Implementation of Government-Mandated Disease Control and Eradication Programs serves as the second primary driver, institutionalizing regular screening as a standard operational requirement. These frameworks often link herd health status to market access, compelling farmers to adhere to strict testing schedules to maintain trade certifications. According to Preventive Veterinary Medicine, August 2024, in the 'Changes in the Johne’s Disease situation in GB dairy herds over 10 years' article, surveillance data revealed that the 25% of herds with the highest average test values accounted for 42% of all positive tests, underscoring the role of centralized schemes in targeting high-risk reservoirs. Additionally, these programs encourage broader management protocols; according to the Journal of Dairy Science, in 2024, integrating young stock into testing strategies could reduce disease transmission by up to 30%, further fueling the global demand for diagnostic consumables.

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Key Market Challenges

The limited sensitivity of current diagnostic tools during the lengthy subclinical phase of the disease constitutes a substantial barrier to the expansion of the Global Johne’s Disease Diagnostics Market. Because infected livestock often harbor Mycobacterium avium subspecies paratuberculosis for years without shedding detectable bacterial loads or generating sufficient antibody responses, standard assays frequently yield false-negative results. This technical inability to reliably identify early-stage carriers undermines producer confidence in the cost-effectiveness of surveillance programs. When substantial investments in screening fail to prevent subsequent outbreaks due to undetected spreaders, livestock owners become hesitant to commit to the recurring testing regimes that drive market revenue.

This skepticism is reinforced by data illustrating low detection rates in managed populations. According to the Action Group on Johne’s, in 2024, the mean prevalence of milk ELISA test-positive results in monitored herds within the United Kingdom was recorded at 2.7%. While this figure indicates managed control, it also highlights the difficulty diagnostics face in uncovering the wider pool of subclinical infection, thereby discouraging the universal adoption of aggressive screening protocols across cost-sensitive operations.

Key Market Trends

The Emergence of AI-Powered MicroRNA Biomarker Technologies is transforming the Global Johne’s Disease Diagnostics Market by addressing the sensitivity gaps of traditional serology. Unlike ELISA tests that rely on delayed antibody production, microRNA profiling analyzes host-response gene expression to identify subclinical carriers before they become infectious. This molecular approach, utilizing machine learning algorithms, allows for the precise categorization of early infection stages that standard assays fail to capture. According to DVM360, May 2025, in the 'New diagnostic approach may help detect Johne disease in cattle earlier' article, a newly validated AI-driven microRNA diagnostic model demonstrated an accuracy of 71.8% in detecting Stage I and II infections, offering a critical advantage in identifying silent spreaders to break transmission cycles.

Concurrently, the Integration of Diagnostic Testing with Cloud-Based Herd Management Platforms is shifting the sector from reactive diagnosis to predictive risk stratification. Modern software solutions now aggregate historical testing data to generate dynamic risk scores, allowing producers to anticipate disease progression rather than merely reacting to positive results. This digital transition facilitates algorithmic clustering to identify high-risk animals that standard protocols might miss. According to Farmers Weekly, February 2025, in the 'Johne’s risk detection improved with new data analysis tool' article, predictive analytics revealed that animals in specific high-risk data clusters were up to 1,000 times more likely to progress to infectious status than their low-risk counterparts, enabling proactive biosecurity decisions.

Segmental Insights

The Enzyme-Linked Immunosorbent Assay (ELISA) segment constitutes the fastest-growing category in the Global Johne’s Disease Diagnostics Market, largely driven by its superior cost-effectiveness and suitability for mass herd screening. In contrast to time-consuming culture methods or more expensive molecular tests, ELISA offers rapid turnaround times that are essential for regular surveillance and early risk assessment in large populations. This practicality aligns with herd health protocols promoted by agencies such as the USDA, which often utilize serological testing for initial monitoring in disease control programs. Consequently, the increasing adoption of these scalable diagnostic tools by livestock producers to minimize economic losses fuels the segment's robust expansion.

Regional Insights

North America holds the leading position in the Global Johne’s Disease Diagnostics Market, primarily driven by a well-established animal healthcare sector and proactive government measures. The region benefits from stringent disease management protocols, such as the control programs overseen by the United States Department of Agriculture (USDA), which promote regular screening and herd management to mitigate infection risks. Additionally, the strong dairy and livestock industries in the United States and Canada maintain high awareness regarding the economic impact of paratuberculosis. This focus on preventive veterinary care ensures consistent demand for diagnostic testing services across the region.

Recent Developments

• In May 2025, MI:RNA Diagnostics announced a strategic collaboration with Scotland’s Rural College (SRUC) to advance the early diagnosis of Johne’s disease. The partnership released results from a preliminary study that utilized artificial intelligence and microRNA profiling to detect the disease with greater accuracy than conventional methods. The study demonstrated that specific microRNA biomarkers in cattle blood could identify infection at early stages, addressing the limitations of current diagnostic sensitivity. This innovative approach aims to provide farmers with a more reliable tool for managing "silent" carriers in their herds, potentially reducing the significant economic losses associated with the disease in the global livestock sector.
• In January 2025, Indical Bioscience issued a notification confirming that its IndiMag Pathogen Kits and Cartridges received approval from the National Animal Health Laboratory Network (NAHLN) for use in specific disease surveillance programs. Coinciding with the release of its 2025 Diagnostics Portfolio Catalogue, the company introduced the new IndiMag Pathogen IM2 Cartridge, designed to enhance the automated isolation of viral RNA and bacterial DNA. These advancements support Indical’s comprehensive bovine workflow, which includes qPCR and ELISA solutions for the detection of Mycobacterium avium subsp. paratuberculosis (MAP), thereby strengthening the infrastructure for high-throughput Johne’s disease testing in veterinary laboratories.
• In November 2024, IDEXX Laboratories and Royal GD participated in the Action Johne’s Conference, a key industry event focused on the control of Mycobacterium avium subsp. paratuberculosis (MAP). During the conference, IDEXX Laboratories presented field experience data regarding the performance of the IDEXX Johne’s Disease ELISA Test, highlighting its utility in herd management and disease surveillance. Royal GD, a leading Dutch animal health organization, provided a comprehensive survey of the international control landscape for the disease. The event emphasized the importance of diagnostic tools in the Phase III implementation of the National Johne’s Management Plan, aiming to improve cattle health and farm profitability through robust testing and risk assessment strategies.
• In October 2024, PBD Biotech presented breakthrough data at the 7th Congress of the European Association of Veterinary Laboratory Diagnosticians (EAVLD) in Padova, Italy. The company unveiled findings demonstrating that its Actiphage diagnostic system could detect live Mycobacterium avium subsp. paratuberculosis (MAP) in the blood of day-old calves. This development addresses a significant gap in the Global Johne’s Disease Diagnostics Market, as traditional tests often fail to identify the infection in young animals due to the long latency period of the disease. The study showed that Actiphage identified infected calves months before they began shedding bacteria in feces, offering a potential solution for breaking the chain of transmission in dairy herds.

Key Market Players

• Zoetis Inc.
• Thermo Fisher Scientific, Inc.
• PBD Biotech Ltd
• IDEXX Laboratories, Inc.
• Innovative Diagnostics SAS
• VMRD Inc.
• BIOMÉRIEUX S.A.
• INDICAL BIOSCIENCE GmbH
• Tetracore, Inc.
• Surefarm Ltd

By Animal Type	By Test Type	By End User	By Region
Cattle Goat Sheep	ELISA PCR AGID Others	Veterinary Hospitals & Clinics Others	North America Europe Asia Pacific South America Middle East & Africa

Report Scope:

In this report, the Global Johne’s Disease Diagnostics Market has been segmented into the following categories, in addition to the industry trends which have also been detailed below:

• Johne’s Disease Diagnostics Market, By Animal Type:

• Cattle
• Goat
• Sheep

• Johne’s Disease Diagnostics Market, By Test Type:

• ELISA
• PCR
• AGID
• Others

• Johne’s Disease Diagnostics Market, By End User:

• Veterinary Hospitals & Clinics
• Others

• Johne’s Disease Diagnostics Market, By Region:

• North America
• United States
• Canada
• Mexico
• Europe
• France
• United Kingdom
• Italy
• Germany
• Spain
• Asia Pacific
• China
• India
• Japan
• Australia
• South Korea
• South America
• Brazil
• Argentina
• Colombia
• Middle East & Africa
• South Africa
• Saudi Arabia
• UAE